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c albicans reference strain sc5314  (ATCC)


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    ATCC c albicans reference strain sc5314
    C Albicans Reference Strain Sc5314, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 585 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c+albicans+reference+strain+sc5314/Candida+albicans+(Robin)+Berkhout/pm41898717-158-0-7
    Average 98 stars, based on 585 article reviews
    c albicans reference strain sc5314 - by Bioz Stars, 2026-09
    98/100 stars

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    Vaccines:

    Article Title: Nature-Derived Ferulic Acid Hybrids with Enhanced Antifungal and Antivirulence Activity Against Candida albicans .
    Article Snippet: C. albicans reference strain SC5314 (wild type; ATCC MYA-2876) was used throughout this study [38].. Yeast cells were routinely maintained on Sabouraud Dextrose Agar (SDA) plates and incubated at 37 ◦C for 24 h. For experimental assays, colonies were harvested and resuspended in liquid Sabouraud medium, followed by incubation at 37 ◦C under shaking conditions.Yeast cells were routinely maintained on Sabouraud Dextrose Agar (SDA) plates and incubated at 37 ◦C for 24 h. For experimental assays, colonies were harvested and resuspended in liquid Sabouraud medium, followed by incubation at 37 ◦C under shaking conditions.

    Article Title: Nature-Derived Ferulic Acid Hybrids with Enhanced Antifungal and Antivirulence Activity Against Candida albicans
    Article Snippet: C. albicans reference strain SC5314 (wild type; ATCC MYA-2876) was used throughout this study [ ].. Yeast cells were routinely maintained on Sabouraud Dextrose Agar (SDA) plates and incubated at 37 °C for 24 h. For experimental assays, colonies were harvested and resuspended in liquid Sabouraud medium, followed by incubation at 37 °C under shaking conditions.Yeast cells were routinely maintained on Sabouraud Dextrose Agar (SDA) plates and incubated at 37 °C for 24 h. For experimental assays, colonies were harvested and resuspended in liquid Sabouraud medium, followed by incubation at 37 °C under shaking conditions.

    Article Title: Next-generation Candida albicans vaccine VXV-01 containing recombinant Als3p and Hyr1p antigens for invasive Candida infections.
    Article Snippet: FluoroSpots in the unstimulated wells of each 787 mouse were subtracted from the antigen-stimulated spot counts and graphed.FluoroSpots in the unstimulated wells of each 787 mouse were subtracted from the antigen-stimulated spot counts and graphed.. 788 Infectious Inoculum Preparation 789 The C. albicans reference strain SC5314 (ATCC- MYA-2876) and C. auris strain 790 CAU-09 (South Asian clade, bronchoalveolar lavage [BAL]) were used in this AR TIC LE IN PR ES S 791 study.. These strains were grown in Yeast Extract Peptone Dextrose (YPD) broth 792 overnight at 30°C with shaking at 200 rpm.These strains were grown in Yeast Extract Peptone Dextrose (YPD) broth 792 overnight at 30°C with shaking at 200 rpm.

    Article Title: Next-generation Candida albicans vaccine VXV-01 containing recombinant Als3p and Hyr1p antigens for invasive Candida infections
    Article Snippet: The C. albicans reference strain SC5314 (ATCC- MYA-2876) and C. auris strain CAU-09 (South Asian clade, bronchoalveolar lavage [BAL]) were used in this study.. These strains were grown in Yeast Extract Peptone Dextrose (YPD) broth overnight at 30 °C with shaking at 200 rpm.These strains were grown in Yeast Extract Peptone Dextrose (YPD) broth overnight at 30 °C with shaking at 200 rpm.

    Article Title: Monoclonal antibodies targeting Candida disrupt biofilms and inhibit growth across global clinical isolates
    Article Snippet: Candida auris clinical isolates AR-0381 (CAU-01), AR-0382 (CAU-02), AR-0383 (CAU-03), AR-0384 (CAU-04), AR-0385 (CAU-05), AR-0386 (CAU-06), AR-0389 (CAU-09) were supplied by the United States Centers for Disease Control and Prevention (CDC, Atlanta, GA, USA).Candida auris clinical isolates AR-0381 (CAU-01), AR-0382 (CAU-02), AR-0383 (CAU-03), AR-0384 (CAU-04), AR-0385 (CAU-05), AR-0386 (CAU-06), AR-0389 (CAU-09) were supplied by the United States Centers for Disease Control and Prevention (CDC, Atlanta, GA, USA).. C. albicans reference strain SC5314 was supplied by the American Type Culture Collection (MYA-2876, ATCC, Manassas, VA, USA).. Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.

    Article Title: Liposomal Fba and Met6 peptide vaccination protects mice from disseminated candidiasis
    Article Snippet: C. auris clinical isolate AR-0389 (CAU-09) was supplied by the U.S. Centers for Disease Control and Prevention (CDC; Atlanta, GA, USA).C. auris clinical isolate AR-0389 (CAU-09) was supplied by the U.S. Centers for Disease Control and Prevention (CDC; Atlanta, GA, USA).. C. albicans reference strain SC5314 was supplied by the American Type Culture Collection (MYA-2876, ATCC, Manassas, VA, USA).. Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.

    Article Title: Liposomal Fba and Met6 peptide vaccination protects mice from disseminated candidiasis.
    Article Snippet: Candida isolates and culture conditions C. auris clinical isolate AR-0389 (CAU-09) was supplied by the U.S. Centers for Disease Control and Prevention (CDC; Atlanta, GA, USA).Candida isolates and culture conditions C. auris clinical isolate AR-0389 (CAU-09) was supplied by the U.S. Centers for Disease Control and Prevention (CDC; Atlanta, GA, USA).. C. albicans reference strain SC5314 was supplied by the American Type Culture Collection (MYA-2876, ATCC, Manassas, VA, USA).. Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.

    Article Title: Candida Cell-Surface-Specific Monoclonal Antibodies Protect Mice against Candida auris Invasive Infection
    Article Snippet: Two antifungal-resistant isolates of C. auris , AR-0386 (CAU-06) and AR-0389 (CAU-09), were supplied by the United States Centers for Disease Control and Prevention (CDC, Atlanta, GA, USA).Two antifungal-resistant isolates of C. auris , AR-0386 (CAU-06) and AR-0389 (CAU-09), were supplied by the United States Centers for Disease Control and Prevention (CDC, Atlanta, GA, USA).. C. albicans reference strain SC5314 was supplied by the American Type Culture Collection (MYA-2876, ATCC, Manassas, VA, USA).. For passive transfer of mAb experiments, the inoculum was serially passaged daily for three days in 25 mL glucose yeast peptide broth at 37 °C and then washed three times in Dulbecco’s phosphate-buffered saline (DPBS).For passive transfer of mAb experiments, the inoculum was serially passaged daily for three days in 25 mL glucose yeast peptide broth at 37 °C and then washed three times in Dulbecco’s phosphate-buffered saline (DPBS).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Nature-Derived Ferulic Acid Hybrids with Enhanced Antifungal and Antivirulence Activity Against Candida albicans .
    Article Snippet: C. albicans reference strain SC5314 (wild type; ATCC MYA-2876) was used throughout this study [38].. Yeast cells were routinely maintained on Sabouraud Dextrose Agar (SDA) plates and incubated at 37 ◦C for 24 h. For experimental assays, colonies were harvested and resuspended in liquid Sabouraud medium, followed by incubation at 37 ◦C under shaking conditions.Yeast cells were routinely maintained on Sabouraud Dextrose Agar (SDA) plates and incubated at 37 ◦C for 24 h. For experimental assays, colonies were harvested and resuspended in liquid Sabouraud medium, followed by incubation at 37 ◦C under shaking conditions.

    Article Title: Nature-Derived Ferulic Acid Hybrids with Enhanced Antifungal and Antivirulence Activity Against Candida albicans
    Article Snippet: C. albicans reference strain SC5314 (wild type; ATCC MYA-2876) was used throughout this study [ ].. Yeast cells were routinely maintained on Sabouraud Dextrose Agar (SDA) plates and incubated at 37 °C for 24 h. For experimental assays, colonies were harvested and resuspended in liquid Sabouraud medium, followed by incubation at 37 °C under shaking conditions.Yeast cells were routinely maintained on Sabouraud Dextrose Agar (SDA) plates and incubated at 37 °C for 24 h. For experimental assays, colonies were harvested and resuspended in liquid Sabouraud medium, followed by incubation at 37 °C under shaking conditions.

    Article Title: Next-generation Candida albicans vaccine VXV-01 containing recombinant Als3p and Hyr1p antigens for invasive Candida infections.
    Article Snippet: FluoroSpots in the unstimulated wells of each 787 mouse were subtracted from the antigen-stimulated spot counts and graphed.FluoroSpots in the unstimulated wells of each 787 mouse were subtracted from the antigen-stimulated spot counts and graphed.. 788 Infectious Inoculum Preparation 789 The C. albicans reference strain SC5314 (ATCC- MYA-2876) and C. auris strain 790 CAU-09 (South Asian clade, bronchoalveolar lavage [BAL]) were used in this AR TIC LE IN PR ES S 791 study.. These strains were grown in Yeast Extract Peptone Dextrose (YPD) broth 792 overnight at 30°C with shaking at 200 rpm.These strains were grown in Yeast Extract Peptone Dextrose (YPD) broth 792 overnight at 30°C with shaking at 200 rpm.

    Article Title: Next-generation Candida albicans vaccine VXV-01 containing recombinant Als3p and Hyr1p antigens for invasive Candida infections
    Article Snippet: The C. albicans reference strain SC5314 (ATCC- MYA-2876) and C. auris strain CAU-09 (South Asian clade, bronchoalveolar lavage [BAL]) were used in this study.. These strains were grown in Yeast Extract Peptone Dextrose (YPD) broth overnight at 30 °C with shaking at 200 rpm.These strains were grown in Yeast Extract Peptone Dextrose (YPD) broth overnight at 30 °C with shaking at 200 rpm.

    Article Title: Monoclonal antibodies targeting Candida disrupt biofilms and inhibit growth across global clinical isolates
    Article Snippet: Candida auris clinical isolates AR-0381 (CAU-01), AR-0382 (CAU-02), AR-0383 (CAU-03), AR-0384 (CAU-04), AR-0385 (CAU-05), AR-0386 (CAU-06), AR-0389 (CAU-09) were supplied by the United States Centers for Disease Control and Prevention (CDC, Atlanta, GA, USA).Candida auris clinical isolates AR-0381 (CAU-01), AR-0382 (CAU-02), AR-0383 (CAU-03), AR-0384 (CAU-04), AR-0385 (CAU-05), AR-0386 (CAU-06), AR-0389 (CAU-09) were supplied by the United States Centers for Disease Control and Prevention (CDC, Atlanta, GA, USA).. C. albicans reference strain SC5314 was supplied by the American Type Culture Collection (MYA-2876, ATCC, Manassas, VA, USA).. Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.

    Article Title: Liposomal Fba and Met6 peptide vaccination protects mice from disseminated candidiasis
    Article Snippet: C. auris clinical isolate AR-0389 (CAU-09) was supplied by the U.S. Centers for Disease Control and Prevention (CDC; Atlanta, GA, USA).C. auris clinical isolate AR-0389 (CAU-09) was supplied by the U.S. Centers for Disease Control and Prevention (CDC; Atlanta, GA, USA).. C. albicans reference strain SC5314 was supplied by the American Type Culture Collection (MYA-2876, ATCC, Manassas, VA, USA).. Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.

    Article Title: Liposomal Fba and Met6 peptide vaccination protects mice from disseminated candidiasis.
    Article Snippet: Candida isolates and culture conditions C. auris clinical isolate AR-0389 (CAU-09) was supplied by the U.S. Centers for Disease Control and Prevention (CDC; Atlanta, GA, USA).Candida isolates and culture conditions C. auris clinical isolate AR-0389 (CAU-09) was supplied by the U.S. Centers for Disease Control and Prevention (CDC; Atlanta, GA, USA).. C. albicans reference strain SC5314 was supplied by the American Type Culture Collection (MYA-2876, ATCC, Manassas, VA, USA).. Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.

    Article Title: Candida Cell-Surface-Specific Monoclonal Antibodies Protect Mice against Candida auris Invasive Infection
    Article Snippet: Two antifungal-resistant isolates of C. auris , AR-0386 (CAU-06) and AR-0389 (CAU-09), were supplied by the United States Centers for Disease Control and Prevention (CDC, Atlanta, GA, USA).Two antifungal-resistant isolates of C. auris , AR-0386 (CAU-06) and AR-0389 (CAU-09), were supplied by the United States Centers for Disease Control and Prevention (CDC, Atlanta, GA, USA).. C. albicans reference strain SC5314 was supplied by the American Type Culture Collection (MYA-2876, ATCC, Manassas, VA, USA).. For passive transfer of mAb experiments, the inoculum was serially passaged daily for three days in 25 mL glucose yeast peptide broth at 37 °C and then washed three times in Dulbecco’s phosphate-buffered saline (DPBS).For passive transfer of mAb experiments, the inoculum was serially passaged daily for three days in 25 mL glucose yeast peptide broth at 37 °C and then washed three times in Dulbecco’s phosphate-buffered saline (DPBS).

    Transformation Assay:

    Article Title: Nature-Derived Ferulic Acid Hybrids with Enhanced Antifungal and Antivirulence Activity Against Candida albicans .
    Article Snippet: C. albicans reference strain SC5314 (wild type; ATCC MYA-2876) was used throughout this study [38].. Yeast cells were routinely maintained on Sabouraud Dextrose Agar (SDA) plates and incubated at 37 ◦C for 24 h. For experimental assays, colonies were harvested and resuspended in liquid Sabouraud medium, followed by incubation at 37 ◦C under shaking conditions.Yeast cells were routinely maintained on Sabouraud Dextrose Agar (SDA) plates and incubated at 37 ◦C for 24 h. For experimental assays, colonies were harvested and resuspended in liquid Sabouraud medium, followed by incubation at 37 ◦C under shaking conditions.

    Article Title: Nature-Derived Ferulic Acid Hybrids with Enhanced Antifungal and Antivirulence Activity Against Candida albicans
    Article Snippet: C. albicans reference strain SC5314 (wild type; ATCC MYA-2876) was used throughout this study [ ].. Yeast cells were routinely maintained on Sabouraud Dextrose Agar (SDA) plates and incubated at 37 °C for 24 h. For experimental assays, colonies were harvested and resuspended in liquid Sabouraud medium, followed by incubation at 37 °C under shaking conditions.Yeast cells were routinely maintained on Sabouraud Dextrose Agar (SDA) plates and incubated at 37 °C for 24 h. For experimental assays, colonies were harvested and resuspended in liquid Sabouraud medium, followed by incubation at 37 °C under shaking conditions.

    Article Title: Next-generation Candida albicans vaccine VXV-01 containing recombinant Als3p and Hyr1p antigens for invasive Candida infections.
    Article Snippet: FluoroSpots in the unstimulated wells of each 787 mouse were subtracted from the antigen-stimulated spot counts and graphed.FluoroSpots in the unstimulated wells of each 787 mouse were subtracted from the antigen-stimulated spot counts and graphed.. 788 Infectious Inoculum Preparation 789 The C. albicans reference strain SC5314 (ATCC- MYA-2876) and C. auris strain 790 CAU-09 (South Asian clade, bronchoalveolar lavage [BAL]) were used in this AR TIC LE IN PR ES S 791 study.. These strains were grown in Yeast Extract Peptone Dextrose (YPD) broth 792 overnight at 30°C with shaking at 200 rpm.These strains were grown in Yeast Extract Peptone Dextrose (YPD) broth 792 overnight at 30°C with shaking at 200 rpm.

    Article Title: Next-generation Candida albicans vaccine VXV-01 containing recombinant Als3p and Hyr1p antigens for invasive Candida infections
    Article Snippet: The C. albicans reference strain SC5314 (ATCC- MYA-2876) and C. auris strain CAU-09 (South Asian clade, bronchoalveolar lavage [BAL]) were used in this study.. These strains were grown in Yeast Extract Peptone Dextrose (YPD) broth overnight at 30 °C with shaking at 200 rpm.These strains were grown in Yeast Extract Peptone Dextrose (YPD) broth overnight at 30 °C with shaking at 200 rpm.

    Article Title: Monoclonal antibodies targeting Candida disrupt biofilms and inhibit growth across global clinical isolates
    Article Snippet: Candida auris clinical isolates AR-0381 (CAU-01), AR-0382 (CAU-02), AR-0383 (CAU-03), AR-0384 (CAU-04), AR-0385 (CAU-05), AR-0386 (CAU-06), AR-0389 (CAU-09) were supplied by the United States Centers for Disease Control and Prevention (CDC, Atlanta, GA, USA).Candida auris clinical isolates AR-0381 (CAU-01), AR-0382 (CAU-02), AR-0383 (CAU-03), AR-0384 (CAU-04), AR-0385 (CAU-05), AR-0386 (CAU-06), AR-0389 (CAU-09) were supplied by the United States Centers for Disease Control and Prevention (CDC, Atlanta, GA, USA).. C. albicans reference strain SC5314 was supplied by the American Type Culture Collection (MYA-2876, ATCC, Manassas, VA, USA).. Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.

    Article Title: Liposomal Fba and Met6 peptide vaccination protects mice from disseminated candidiasis
    Article Snippet: C. auris clinical isolate AR-0389 (CAU-09) was supplied by the U.S. Centers for Disease Control and Prevention (CDC; Atlanta, GA, USA).C. auris clinical isolate AR-0389 (CAU-09) was supplied by the U.S. Centers for Disease Control and Prevention (CDC; Atlanta, GA, USA).. C. albicans reference strain SC5314 was supplied by the American Type Culture Collection (MYA-2876, ATCC, Manassas, VA, USA).. Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.

    Article Title: Liposomal Fba and Met6 peptide vaccination protects mice from disseminated candidiasis.
    Article Snippet: Candida isolates and culture conditions C. auris clinical isolate AR-0389 (CAU-09) was supplied by the U.S. Centers for Disease Control and Prevention (CDC; Atlanta, GA, USA).Candida isolates and culture conditions C. auris clinical isolate AR-0389 (CAU-09) was supplied by the U.S. Centers for Disease Control and Prevention (CDC; Atlanta, GA, USA).. C. albicans reference strain SC5314 was supplied by the American Type Culture Collection (MYA-2876, ATCC, Manassas, VA, USA).. Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.

    Article Title: Candida Cell-Surface-Specific Monoclonal Antibodies Protect Mice against Candida auris Invasive Infection
    Article Snippet: Two antifungal-resistant isolates of C. auris , AR-0386 (CAU-06) and AR-0389 (CAU-09), were supplied by the United States Centers for Disease Control and Prevention (CDC, Atlanta, GA, USA).Two antifungal-resistant isolates of C. auris , AR-0386 (CAU-06) and AR-0389 (CAU-09), were supplied by the United States Centers for Disease Control and Prevention (CDC, Atlanta, GA, USA).. C. albicans reference strain SC5314 was supplied by the American Type Culture Collection (MYA-2876, ATCC, Manassas, VA, USA).. For passive transfer of mAb experiments, the inoculum was serially passaged daily for three days in 25 mL glucose yeast peptide broth at 37 °C and then washed three times in Dulbecco’s phosphate-buffered saline (DPBS).For passive transfer of mAb experiments, the inoculum was serially passaged daily for three days in 25 mL glucose yeast peptide broth at 37 °C and then washed three times in Dulbecco’s phosphate-buffered saline (DPBS).

    Adjuvant:

    Article Title: Nature-Derived Ferulic Acid Hybrids with Enhanced Antifungal and Antivirulence Activity Against Candida albicans .
    Article Snippet: C. albicans reference strain SC5314 (wild type; ATCC MYA-2876) was used throughout this study [38].. Yeast cells were routinely maintained on Sabouraud Dextrose Agar (SDA) plates and incubated at 37 ◦C for 24 h. For experimental assays, colonies were harvested and resuspended in liquid Sabouraud medium, followed by incubation at 37 ◦C under shaking conditions.Yeast cells were routinely maintained on Sabouraud Dextrose Agar (SDA) plates and incubated at 37 ◦C for 24 h. For experimental assays, colonies were harvested and resuspended in liquid Sabouraud medium, followed by incubation at 37 ◦C under shaking conditions.

    Article Title: Nature-Derived Ferulic Acid Hybrids with Enhanced Antifungal and Antivirulence Activity Against Candida albicans
    Article Snippet: C. albicans reference strain SC5314 (wild type; ATCC MYA-2876) was used throughout this study [ ].. Yeast cells were routinely maintained on Sabouraud Dextrose Agar (SDA) plates and incubated at 37 °C for 24 h. For experimental assays, colonies were harvested and resuspended in liquid Sabouraud medium, followed by incubation at 37 °C under shaking conditions.Yeast cells were routinely maintained on Sabouraud Dextrose Agar (SDA) plates and incubated at 37 °C for 24 h. For experimental assays, colonies were harvested and resuspended in liquid Sabouraud medium, followed by incubation at 37 °C under shaking conditions.

    Article Title: Next-generation Candida albicans vaccine VXV-01 containing recombinant Als3p and Hyr1p antigens for invasive Candida infections.
    Article Snippet: FluoroSpots in the unstimulated wells of each 787 mouse were subtracted from the antigen-stimulated spot counts and graphed.FluoroSpots in the unstimulated wells of each 787 mouse were subtracted from the antigen-stimulated spot counts and graphed.. 788 Infectious Inoculum Preparation 789 The C. albicans reference strain SC5314 (ATCC- MYA-2876) and C. auris strain 790 CAU-09 (South Asian clade, bronchoalveolar lavage [BAL]) were used in this AR TIC LE IN PR ES S 791 study.. These strains were grown in Yeast Extract Peptone Dextrose (YPD) broth 792 overnight at 30°C with shaking at 200 rpm.These strains were grown in Yeast Extract Peptone Dextrose (YPD) broth 792 overnight at 30°C with shaking at 200 rpm.

    Article Title: Next-generation Candida albicans vaccine VXV-01 containing recombinant Als3p and Hyr1p antigens for invasive Candida infections
    Article Snippet: The C. albicans reference strain SC5314 (ATCC- MYA-2876) and C. auris strain CAU-09 (South Asian clade, bronchoalveolar lavage [BAL]) were used in this study.. These strains were grown in Yeast Extract Peptone Dextrose (YPD) broth overnight at 30 °C with shaking at 200 rpm.These strains were grown in Yeast Extract Peptone Dextrose (YPD) broth overnight at 30 °C with shaking at 200 rpm.

    Article Title: Monoclonal antibodies targeting Candida disrupt biofilms and inhibit growth across global clinical isolates
    Article Snippet: Candida auris clinical isolates AR-0381 (CAU-01), AR-0382 (CAU-02), AR-0383 (CAU-03), AR-0384 (CAU-04), AR-0385 (CAU-05), AR-0386 (CAU-06), AR-0389 (CAU-09) were supplied by the United States Centers for Disease Control and Prevention (CDC, Atlanta, GA, USA).Candida auris clinical isolates AR-0381 (CAU-01), AR-0382 (CAU-02), AR-0383 (CAU-03), AR-0384 (CAU-04), AR-0385 (CAU-05), AR-0386 (CAU-06), AR-0389 (CAU-09) were supplied by the United States Centers for Disease Control and Prevention (CDC, Atlanta, GA, USA).. C. albicans reference strain SC5314 was supplied by the American Type Culture Collection (MYA-2876, ATCC, Manassas, VA, USA).. Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.

    Article Title: Liposomal Fba and Met6 peptide vaccination protects mice from disseminated candidiasis
    Article Snippet: C. auris clinical isolate AR-0389 (CAU-09) was supplied by the U.S. Centers for Disease Control and Prevention (CDC; Atlanta, GA, USA).C. auris clinical isolate AR-0389 (CAU-09) was supplied by the U.S. Centers for Disease Control and Prevention (CDC; Atlanta, GA, USA).. C. albicans reference strain SC5314 was supplied by the American Type Culture Collection (MYA-2876, ATCC, Manassas, VA, USA).. Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.

    Article Title: Liposomal Fba and Met6 peptide vaccination protects mice from disseminated candidiasis.
    Article Snippet: Candida isolates and culture conditions C. auris clinical isolate AR-0389 (CAU-09) was supplied by the U.S. Centers for Disease Control and Prevention (CDC; Atlanta, GA, USA).Candida isolates and culture conditions C. auris clinical isolate AR-0389 (CAU-09) was supplied by the U.S. Centers for Disease Control and Prevention (CDC; Atlanta, GA, USA).. C. albicans reference strain SC5314 was supplied by the American Type Culture Collection (MYA-2876, ATCC, Manassas, VA, USA).. Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.

    Article Title: Candida Cell-Surface-Specific Monoclonal Antibodies Protect Mice against Candida auris Invasive Infection
    Article Snippet: Two antifungal-resistant isolates of C. auris , AR-0386 (CAU-06) and AR-0389 (CAU-09), were supplied by the United States Centers for Disease Control and Prevention (CDC, Atlanta, GA, USA).Two antifungal-resistant isolates of C. auris , AR-0386 (CAU-06) and AR-0389 (CAU-09), were supplied by the United States Centers for Disease Control and Prevention (CDC, Atlanta, GA, USA).. C. albicans reference strain SC5314 was supplied by the American Type Culture Collection (MYA-2876, ATCC, Manassas, VA, USA).. For passive transfer of mAb experiments, the inoculum was serially passaged daily for three days in 25 mL glucose yeast peptide broth at 37 °C and then washed three times in Dulbecco’s phosphate-buffered saline (DPBS).For passive transfer of mAb experiments, the inoculum was serially passaged daily for three days in 25 mL glucose yeast peptide broth at 37 °C and then washed three times in Dulbecco’s phosphate-buffered saline (DPBS).

    Binding Assay:

    Article Title: Nature-Derived Ferulic Acid Hybrids with Enhanced Antifungal and Antivirulence Activity Against Candida albicans .
    Article Snippet: C. albicans reference strain SC5314 (wild type; ATCC MYA-2876) was used throughout this study [38].. Yeast cells were routinely maintained on Sabouraud Dextrose Agar (SDA) plates and incubated at 37 ◦C for 24 h. For experimental assays, colonies were harvested and resuspended in liquid Sabouraud medium, followed by incubation at 37 ◦C under shaking conditions.Yeast cells were routinely maintained on Sabouraud Dextrose Agar (SDA) plates and incubated at 37 ◦C for 24 h. For experimental assays, colonies were harvested and resuspended in liquid Sabouraud medium, followed by incubation at 37 ◦C under shaking conditions.

    Article Title: Nature-Derived Ferulic Acid Hybrids with Enhanced Antifungal and Antivirulence Activity Against Candida albicans
    Article Snippet: C. albicans reference strain SC5314 (wild type; ATCC MYA-2876) was used throughout this study [ ].. Yeast cells were routinely maintained on Sabouraud Dextrose Agar (SDA) plates and incubated at 37 °C for 24 h. For experimental assays, colonies were harvested and resuspended in liquid Sabouraud medium, followed by incubation at 37 °C under shaking conditions.Yeast cells were routinely maintained on Sabouraud Dextrose Agar (SDA) plates and incubated at 37 °C for 24 h. For experimental assays, colonies were harvested and resuspended in liquid Sabouraud medium, followed by incubation at 37 °C under shaking conditions.

    Article Title: Next-generation Candida albicans vaccine VXV-01 containing recombinant Als3p and Hyr1p antigens for invasive Candida infections.
    Article Snippet: FluoroSpots in the unstimulated wells of each 787 mouse were subtracted from the antigen-stimulated spot counts and graphed.FluoroSpots in the unstimulated wells of each 787 mouse were subtracted from the antigen-stimulated spot counts and graphed.. 788 Infectious Inoculum Preparation 789 The C. albicans reference strain SC5314 (ATCC- MYA-2876) and C. auris strain 790 CAU-09 (South Asian clade, bronchoalveolar lavage [BAL]) were used in this AR TIC LE IN PR ES S 791 study.. These strains were grown in Yeast Extract Peptone Dextrose (YPD) broth 792 overnight at 30°C with shaking at 200 rpm.These strains were grown in Yeast Extract Peptone Dextrose (YPD) broth 792 overnight at 30°C with shaking at 200 rpm.

    Article Title: Next-generation Candida albicans vaccine VXV-01 containing recombinant Als3p and Hyr1p antigens for invasive Candida infections
    Article Snippet: The C. albicans reference strain SC5314 (ATCC- MYA-2876) and C. auris strain CAU-09 (South Asian clade, bronchoalveolar lavage [BAL]) were used in this study.. These strains were grown in Yeast Extract Peptone Dextrose (YPD) broth overnight at 30 °C with shaking at 200 rpm.These strains were grown in Yeast Extract Peptone Dextrose (YPD) broth overnight at 30 °C with shaking at 200 rpm.

    Article Title: Monoclonal antibodies targeting Candida disrupt biofilms and inhibit growth across global clinical isolates
    Article Snippet: Candida auris clinical isolates AR-0381 (CAU-01), AR-0382 (CAU-02), AR-0383 (CAU-03), AR-0384 (CAU-04), AR-0385 (CAU-05), AR-0386 (CAU-06), AR-0389 (CAU-09) were supplied by the United States Centers for Disease Control and Prevention (CDC, Atlanta, GA, USA).Candida auris clinical isolates AR-0381 (CAU-01), AR-0382 (CAU-02), AR-0383 (CAU-03), AR-0384 (CAU-04), AR-0385 (CAU-05), AR-0386 (CAU-06), AR-0389 (CAU-09) were supplied by the United States Centers for Disease Control and Prevention (CDC, Atlanta, GA, USA).. C. albicans reference strain SC5314 was supplied by the American Type Culture Collection (MYA-2876, ATCC, Manassas, VA, USA).. Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.

    Article Title: Liposomal Fba and Met6 peptide vaccination protects mice from disseminated candidiasis
    Article Snippet: C. auris clinical isolate AR-0389 (CAU-09) was supplied by the U.S. Centers for Disease Control and Prevention (CDC; Atlanta, GA, USA).C. auris clinical isolate AR-0389 (CAU-09) was supplied by the U.S. Centers for Disease Control and Prevention (CDC; Atlanta, GA, USA).. C. albicans reference strain SC5314 was supplied by the American Type Culture Collection (MYA-2876, ATCC, Manassas, VA, USA).. Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.

    Article Title: Liposomal Fba and Met6 peptide vaccination protects mice from disseminated candidiasis.
    Article Snippet: Candida isolates and culture conditions C. auris clinical isolate AR-0389 (CAU-09) was supplied by the U.S. Centers for Disease Control and Prevention (CDC; Atlanta, GA, USA).Candida isolates and culture conditions C. auris clinical isolate AR-0389 (CAU-09) was supplied by the U.S. Centers for Disease Control and Prevention (CDC; Atlanta, GA, USA).. C. albicans reference strain SC5314 was supplied by the American Type Culture Collection (MYA-2876, ATCC, Manassas, VA, USA).. Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.

    Article Title: Candida Cell-Surface-Specific Monoclonal Antibodies Protect Mice against Candida auris Invasive Infection
    Article Snippet: Two antifungal-resistant isolates of C. auris , AR-0386 (CAU-06) and AR-0389 (CAU-09), were supplied by the United States Centers for Disease Control and Prevention (CDC, Atlanta, GA, USA).Two antifungal-resistant isolates of C. auris , AR-0386 (CAU-06) and AR-0389 (CAU-09), were supplied by the United States Centers for Disease Control and Prevention (CDC, Atlanta, GA, USA).. C. albicans reference strain SC5314 was supplied by the American Type Culture Collection (MYA-2876, ATCC, Manassas, VA, USA).. For passive transfer of mAb experiments, the inoculum was serially passaged daily for three days in 25 mL glucose yeast peptide broth at 37 °C and then washed three times in Dulbecco’s phosphate-buffered saline (DPBS).For passive transfer of mAb experiments, the inoculum was serially passaged daily for three days in 25 mL glucose yeast peptide broth at 37 °C and then washed three times in Dulbecco’s phosphate-buffered saline (DPBS).

    Bioprocessing:

    Article Title: Nature-Derived Ferulic Acid Hybrids with Enhanced Antifungal and Antivirulence Activity Against Candida albicans .
    Article Snippet: C. albicans reference strain SC5314 (wild type; ATCC MYA-2876) was used throughout this study [38].. Yeast cells were routinely maintained on Sabouraud Dextrose Agar (SDA) plates and incubated at 37 ◦C for 24 h. For experimental assays, colonies were harvested and resuspended in liquid Sabouraud medium, followed by incubation at 37 ◦C under shaking conditions.Yeast cells were routinely maintained on Sabouraud Dextrose Agar (SDA) plates and incubated at 37 ◦C for 24 h. For experimental assays, colonies were harvested and resuspended in liquid Sabouraud medium, followed by incubation at 37 ◦C under shaking conditions.

    Article Title: Nature-Derived Ferulic Acid Hybrids with Enhanced Antifungal and Antivirulence Activity Against Candida albicans
    Article Snippet: C. albicans reference strain SC5314 (wild type; ATCC MYA-2876) was used throughout this study [ ].. Yeast cells were routinely maintained on Sabouraud Dextrose Agar (SDA) plates and incubated at 37 °C for 24 h. For experimental assays, colonies were harvested and resuspended in liquid Sabouraud medium, followed by incubation at 37 °C under shaking conditions.Yeast cells were routinely maintained on Sabouraud Dextrose Agar (SDA) plates and incubated at 37 °C for 24 h. For experimental assays, colonies were harvested and resuspended in liquid Sabouraud medium, followed by incubation at 37 °C under shaking conditions.

    Article Title: Next-generation Candida albicans vaccine VXV-01 containing recombinant Als3p and Hyr1p antigens for invasive Candida infections.
    Article Snippet: FluoroSpots in the unstimulated wells of each 787 mouse were subtracted from the antigen-stimulated spot counts and graphed.FluoroSpots in the unstimulated wells of each 787 mouse were subtracted from the antigen-stimulated spot counts and graphed.. 788 Infectious Inoculum Preparation 789 The C. albicans reference strain SC5314 (ATCC- MYA-2876) and C. auris strain 790 CAU-09 (South Asian clade, bronchoalveolar lavage [BAL]) were used in this AR TIC LE IN PR ES S 791 study.. These strains were grown in Yeast Extract Peptone Dextrose (YPD) broth 792 overnight at 30°C with shaking at 200 rpm.These strains were grown in Yeast Extract Peptone Dextrose (YPD) broth 792 overnight at 30°C with shaking at 200 rpm.

    Article Title: Next-generation Candida albicans vaccine VXV-01 containing recombinant Als3p and Hyr1p antigens for invasive Candida infections
    Article Snippet: The C. albicans reference strain SC5314 (ATCC- MYA-2876) and C. auris strain CAU-09 (South Asian clade, bronchoalveolar lavage [BAL]) were used in this study.. These strains were grown in Yeast Extract Peptone Dextrose (YPD) broth overnight at 30 °C with shaking at 200 rpm.These strains were grown in Yeast Extract Peptone Dextrose (YPD) broth overnight at 30 °C with shaking at 200 rpm.

    Article Title: Monoclonal antibodies targeting Candida disrupt biofilms and inhibit growth across global clinical isolates
    Article Snippet: Candida auris clinical isolates AR-0381 (CAU-01), AR-0382 (CAU-02), AR-0383 (CAU-03), AR-0384 (CAU-04), AR-0385 (CAU-05), AR-0386 (CAU-06), AR-0389 (CAU-09) were supplied by the United States Centers for Disease Control and Prevention (CDC, Atlanta, GA, USA).Candida auris clinical isolates AR-0381 (CAU-01), AR-0382 (CAU-02), AR-0383 (CAU-03), AR-0384 (CAU-04), AR-0385 (CAU-05), AR-0386 (CAU-06), AR-0389 (CAU-09) were supplied by the United States Centers for Disease Control and Prevention (CDC, Atlanta, GA, USA).. C. albicans reference strain SC5314 was supplied by the American Type Culture Collection (MYA-2876, ATCC, Manassas, VA, USA).. Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.

    Article Title: Liposomal Fba and Met6 peptide vaccination protects mice from disseminated candidiasis
    Article Snippet: C. auris clinical isolate AR-0389 (CAU-09) was supplied by the U.S. Centers for Disease Control and Prevention (CDC; Atlanta, GA, USA).C. auris clinical isolate AR-0389 (CAU-09) was supplied by the U.S. Centers for Disease Control and Prevention (CDC; Atlanta, GA, USA).. C. albicans reference strain SC5314 was supplied by the American Type Culture Collection (MYA-2876, ATCC, Manassas, VA, USA).. Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.

    Article Title: Liposomal Fba and Met6 peptide vaccination protects mice from disseminated candidiasis.
    Article Snippet: Candida isolates and culture conditions C. auris clinical isolate AR-0389 (CAU-09) was supplied by the U.S. Centers for Disease Control and Prevention (CDC; Atlanta, GA, USA).Candida isolates and culture conditions C. auris clinical isolate AR-0389 (CAU-09) was supplied by the U.S. Centers for Disease Control and Prevention (CDC; Atlanta, GA, USA).. C. albicans reference strain SC5314 was supplied by the American Type Culture Collection (MYA-2876, ATCC, Manassas, VA, USA).. Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.

    Article Title: Candida Cell-Surface-Specific Monoclonal Antibodies Protect Mice against Candida auris Invasive Infection
    Article Snippet: Two antifungal-resistant isolates of C. auris , AR-0386 (CAU-06) and AR-0389 (CAU-09), were supplied by the United States Centers for Disease Control and Prevention (CDC, Atlanta, GA, USA).Two antifungal-resistant isolates of C. auris , AR-0386 (CAU-06) and AR-0389 (CAU-09), were supplied by the United States Centers for Disease Control and Prevention (CDC, Atlanta, GA, USA).. C. albicans reference strain SC5314 was supplied by the American Type Culture Collection (MYA-2876, ATCC, Manassas, VA, USA).. For passive transfer of mAb experiments, the inoculum was serially passaged daily for three days in 25 mL glucose yeast peptide broth at 37 °C and then washed three times in Dulbecco’s phosphate-buffered saline (DPBS).For passive transfer of mAb experiments, the inoculum was serially passaged daily for three days in 25 mL glucose yeast peptide broth at 37 °C and then washed three times in Dulbecco’s phosphate-buffered saline (DPBS).

    Flow Cytometry:

    Article Title: Nature-Derived Ferulic Acid Hybrids with Enhanced Antifungal and Antivirulence Activity Against Candida albicans .
    Article Snippet: C. albicans reference strain SC5314 (wild type; ATCC MYA-2876) was used throughout this study [38].. Yeast cells were routinely maintained on Sabouraud Dextrose Agar (SDA) plates and incubated at 37 ◦C for 24 h. For experimental assays, colonies were harvested and resuspended in liquid Sabouraud medium, followed by incubation at 37 ◦C under shaking conditions.Yeast cells were routinely maintained on Sabouraud Dextrose Agar (SDA) plates and incubated at 37 ◦C for 24 h. For experimental assays, colonies were harvested and resuspended in liquid Sabouraud medium, followed by incubation at 37 ◦C under shaking conditions.

    Article Title: Nature-Derived Ferulic Acid Hybrids with Enhanced Antifungal and Antivirulence Activity Against Candida albicans
    Article Snippet: C. albicans reference strain SC5314 (wild type; ATCC MYA-2876) was used throughout this study [ ].. Yeast cells were routinely maintained on Sabouraud Dextrose Agar (SDA) plates and incubated at 37 °C for 24 h. For experimental assays, colonies were harvested and resuspended in liquid Sabouraud medium, followed by incubation at 37 °C under shaking conditions.Yeast cells were routinely maintained on Sabouraud Dextrose Agar (SDA) plates and incubated at 37 °C for 24 h. For experimental assays, colonies were harvested and resuspended in liquid Sabouraud medium, followed by incubation at 37 °C under shaking conditions.

    Article Title: Next-generation Candida albicans vaccine VXV-01 containing recombinant Als3p and Hyr1p antigens for invasive Candida infections.
    Article Snippet: FluoroSpots in the unstimulated wells of each 787 mouse were subtracted from the antigen-stimulated spot counts and graphed.FluoroSpots in the unstimulated wells of each 787 mouse were subtracted from the antigen-stimulated spot counts and graphed.. 788 Infectious Inoculum Preparation 789 The C. albicans reference strain SC5314 (ATCC- MYA-2876) and C. auris strain 790 CAU-09 (South Asian clade, bronchoalveolar lavage [BAL]) were used in this AR TIC LE IN PR ES S 791 study.. These strains were grown in Yeast Extract Peptone Dextrose (YPD) broth 792 overnight at 30°C with shaking at 200 rpm.These strains were grown in Yeast Extract Peptone Dextrose (YPD) broth 792 overnight at 30°C with shaking at 200 rpm.

    Article Title: Next-generation Candida albicans vaccine VXV-01 containing recombinant Als3p and Hyr1p antigens for invasive Candida infections
    Article Snippet: The C. albicans reference strain SC5314 (ATCC- MYA-2876) and C. auris strain CAU-09 (South Asian clade, bronchoalveolar lavage [BAL]) were used in this study.. These strains were grown in Yeast Extract Peptone Dextrose (YPD) broth overnight at 30 °C with shaking at 200 rpm.These strains were grown in Yeast Extract Peptone Dextrose (YPD) broth overnight at 30 °C with shaking at 200 rpm.

    Article Title: Monoclonal antibodies targeting Candida disrupt biofilms and inhibit growth across global clinical isolates
    Article Snippet: Candida auris clinical isolates AR-0381 (CAU-01), AR-0382 (CAU-02), AR-0383 (CAU-03), AR-0384 (CAU-04), AR-0385 (CAU-05), AR-0386 (CAU-06), AR-0389 (CAU-09) were supplied by the United States Centers for Disease Control and Prevention (CDC, Atlanta, GA, USA).Candida auris clinical isolates AR-0381 (CAU-01), AR-0382 (CAU-02), AR-0383 (CAU-03), AR-0384 (CAU-04), AR-0385 (CAU-05), AR-0386 (CAU-06), AR-0389 (CAU-09) were supplied by the United States Centers for Disease Control and Prevention (CDC, Atlanta, GA, USA).. C. albicans reference strain SC5314 was supplied by the American Type Culture Collection (MYA-2876, ATCC, Manassas, VA, USA).. Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.

    Article Title: Liposomal Fba and Met6 peptide vaccination protects mice from disseminated candidiasis
    Article Snippet: C. auris clinical isolate AR-0389 (CAU-09) was supplied by the U.S. Centers for Disease Control and Prevention (CDC; Atlanta, GA, USA).C. auris clinical isolate AR-0389 (CAU-09) was supplied by the U.S. Centers for Disease Control and Prevention (CDC; Atlanta, GA, USA).. C. albicans reference strain SC5314 was supplied by the American Type Culture Collection (MYA-2876, ATCC, Manassas, VA, USA).. Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.

    Article Title: Liposomal Fba and Met6 peptide vaccination protects mice from disseminated candidiasis.
    Article Snippet: Candida isolates and culture conditions C. auris clinical isolate AR-0389 (CAU-09) was supplied by the U.S. Centers for Disease Control and Prevention (CDC; Atlanta, GA, USA).Candida isolates and culture conditions C. auris clinical isolate AR-0389 (CAU-09) was supplied by the U.S. Centers for Disease Control and Prevention (CDC; Atlanta, GA, USA).. C. albicans reference strain SC5314 was supplied by the American Type Culture Collection (MYA-2876, ATCC, Manassas, VA, USA).. Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.

    Article Title: Candida Cell-Surface-Specific Monoclonal Antibodies Protect Mice against Candida auris Invasive Infection
    Article Snippet: Two antifungal-resistant isolates of C. auris , AR-0386 (CAU-06) and AR-0389 (CAU-09), were supplied by the United States Centers for Disease Control and Prevention (CDC, Atlanta, GA, USA).Two antifungal-resistant isolates of C. auris , AR-0386 (CAU-06) and AR-0389 (CAU-09), were supplied by the United States Centers for Disease Control and Prevention (CDC, Atlanta, GA, USA).. C. albicans reference strain SC5314 was supplied by the American Type Culture Collection (MYA-2876, ATCC, Manassas, VA, USA).. For passive transfer of mAb experiments, the inoculum was serially passaged daily for three days in 25 mL glucose yeast peptide broth at 37 °C and then washed three times in Dulbecco’s phosphate-buffered saline (DPBS).For passive transfer of mAb experiments, the inoculum was serially passaged daily for three days in 25 mL glucose yeast peptide broth at 37 °C and then washed three times in Dulbecco’s phosphate-buffered saline (DPBS).

    Expressing:

    Article Title: Nature-Derived Ferulic Acid Hybrids with Enhanced Antifungal and Antivirulence Activity Against Candida albicans .
    Article Snippet: C. albicans reference strain SC5314 (wild type; ATCC MYA-2876) was used throughout this study [38].. Yeast cells were routinely maintained on Sabouraud Dextrose Agar (SDA) plates and incubated at 37 ◦C for 24 h. For experimental assays, colonies were harvested and resuspended in liquid Sabouraud medium, followed by incubation at 37 ◦C under shaking conditions.Yeast cells were routinely maintained on Sabouraud Dextrose Agar (SDA) plates and incubated at 37 ◦C for 24 h. For experimental assays, colonies were harvested and resuspended in liquid Sabouraud medium, followed by incubation at 37 ◦C under shaking conditions.

    Article Title: Nature-Derived Ferulic Acid Hybrids with Enhanced Antifungal and Antivirulence Activity Against Candida albicans
    Article Snippet: C. albicans reference strain SC5314 (wild type; ATCC MYA-2876) was used throughout this study [ ].. Yeast cells were routinely maintained on Sabouraud Dextrose Agar (SDA) plates and incubated at 37 °C for 24 h. For experimental assays, colonies were harvested and resuspended in liquid Sabouraud medium, followed by incubation at 37 °C under shaking conditions.Yeast cells were routinely maintained on Sabouraud Dextrose Agar (SDA) plates and incubated at 37 °C for 24 h. For experimental assays, colonies were harvested and resuspended in liquid Sabouraud medium, followed by incubation at 37 °C under shaking conditions.

    Article Title: Next-generation Candida albicans vaccine VXV-01 containing recombinant Als3p and Hyr1p antigens for invasive Candida infections.
    Article Snippet: FluoroSpots in the unstimulated wells of each 787 mouse were subtracted from the antigen-stimulated spot counts and graphed.FluoroSpots in the unstimulated wells of each 787 mouse were subtracted from the antigen-stimulated spot counts and graphed.. 788 Infectious Inoculum Preparation 789 The C. albicans reference strain SC5314 (ATCC- MYA-2876) and C. auris strain 790 CAU-09 (South Asian clade, bronchoalveolar lavage [BAL]) were used in this AR TIC LE IN PR ES S 791 study.. These strains were grown in Yeast Extract Peptone Dextrose (YPD) broth 792 overnight at 30°C with shaking at 200 rpm.These strains were grown in Yeast Extract Peptone Dextrose (YPD) broth 792 overnight at 30°C with shaking at 200 rpm.

    Article Title: Next-generation Candida albicans vaccine VXV-01 containing recombinant Als3p and Hyr1p antigens for invasive Candida infections
    Article Snippet: The C. albicans reference strain SC5314 (ATCC- MYA-2876) and C. auris strain CAU-09 (South Asian clade, bronchoalveolar lavage [BAL]) were used in this study.. These strains were grown in Yeast Extract Peptone Dextrose (YPD) broth overnight at 30 °C with shaking at 200 rpm.These strains were grown in Yeast Extract Peptone Dextrose (YPD) broth overnight at 30 °C with shaking at 200 rpm.

    Article Title: Monoclonal antibodies targeting Candida disrupt biofilms and inhibit growth across global clinical isolates
    Article Snippet: Candida auris clinical isolates AR-0381 (CAU-01), AR-0382 (CAU-02), AR-0383 (CAU-03), AR-0384 (CAU-04), AR-0385 (CAU-05), AR-0386 (CAU-06), AR-0389 (CAU-09) were supplied by the United States Centers for Disease Control and Prevention (CDC, Atlanta, GA, USA).Candida auris clinical isolates AR-0381 (CAU-01), AR-0382 (CAU-02), AR-0383 (CAU-03), AR-0384 (CAU-04), AR-0385 (CAU-05), AR-0386 (CAU-06), AR-0389 (CAU-09) were supplied by the United States Centers for Disease Control and Prevention (CDC, Atlanta, GA, USA).. C. albicans reference strain SC5314 was supplied by the American Type Culture Collection (MYA-2876, ATCC, Manassas, VA, USA).. Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.

    Article Title: Liposomal Fba and Met6 peptide vaccination protects mice from disseminated candidiasis
    Article Snippet: C. auris clinical isolate AR-0389 (CAU-09) was supplied by the U.S. Centers for Disease Control and Prevention (CDC; Atlanta, GA, USA).C. auris clinical isolate AR-0389 (CAU-09) was supplied by the U.S. Centers for Disease Control and Prevention (CDC; Atlanta, GA, USA).. C. albicans reference strain SC5314 was supplied by the American Type Culture Collection (MYA-2876, ATCC, Manassas, VA, USA).. Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.

    Article Title: Liposomal Fba and Met6 peptide vaccination protects mice from disseminated candidiasis.
    Article Snippet: Candida isolates and culture conditions C. auris clinical isolate AR-0389 (CAU-09) was supplied by the U.S. Centers for Disease Control and Prevention (CDC; Atlanta, GA, USA).Candida isolates and culture conditions C. auris clinical isolate AR-0389 (CAU-09) was supplied by the U.S. Centers for Disease Control and Prevention (CDC; Atlanta, GA, USA).. C. albicans reference strain SC5314 was supplied by the American Type Culture Collection (MYA-2876, ATCC, Manassas, VA, USA).. Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.

    Article Title: Candida Cell-Surface-Specific Monoclonal Antibodies Protect Mice against Candida auris Invasive Infection
    Article Snippet: Two antifungal-resistant isolates of C. auris , AR-0386 (CAU-06) and AR-0389 (CAU-09), were supplied by the United States Centers for Disease Control and Prevention (CDC, Atlanta, GA, USA).Two antifungal-resistant isolates of C. auris , AR-0386 (CAU-06) and AR-0389 (CAU-09), were supplied by the United States Centers for Disease Control and Prevention (CDC, Atlanta, GA, USA).. C. albicans reference strain SC5314 was supplied by the American Type Culture Collection (MYA-2876, ATCC, Manassas, VA, USA).. For passive transfer of mAb experiments, the inoculum was serially passaged daily for three days in 25 mL glucose yeast peptide broth at 37 °C and then washed three times in Dulbecco’s phosphate-buffered saline (DPBS).For passive transfer of mAb experiments, the inoculum was serially passaged daily for three days in 25 mL glucose yeast peptide broth at 37 °C and then washed three times in Dulbecco’s phosphate-buffered saline (DPBS).

    Confocal Microscopy:

    Article Title: Nature-Derived Ferulic Acid Hybrids with Enhanced Antifungal and Antivirulence Activity Against Candida albicans .
    Article Snippet: C. albicans reference strain SC5314 (wild type; ATCC MYA-2876) was used throughout this study [38].. Yeast cells were routinely maintained on Sabouraud Dextrose Agar (SDA) plates and incubated at 37 ◦C for 24 h. For experimental assays, colonies were harvested and resuspended in liquid Sabouraud medium, followed by incubation at 37 ◦C under shaking conditions.Yeast cells were routinely maintained on Sabouraud Dextrose Agar (SDA) plates and incubated at 37 ◦C for 24 h. For experimental assays, colonies were harvested and resuspended in liquid Sabouraud medium, followed by incubation at 37 ◦C under shaking conditions.

    Article Title: Nature-Derived Ferulic Acid Hybrids with Enhanced Antifungal and Antivirulence Activity Against Candida albicans
    Article Snippet: C. albicans reference strain SC5314 (wild type; ATCC MYA-2876) was used throughout this study [ ].. Yeast cells were routinely maintained on Sabouraud Dextrose Agar (SDA) plates and incubated at 37 °C for 24 h. For experimental assays, colonies were harvested and resuspended in liquid Sabouraud medium, followed by incubation at 37 °C under shaking conditions.Yeast cells were routinely maintained on Sabouraud Dextrose Agar (SDA) plates and incubated at 37 °C for 24 h. For experimental assays, colonies were harvested and resuspended in liquid Sabouraud medium, followed by incubation at 37 °C under shaking conditions.

    Article Title: Next-generation Candida albicans vaccine VXV-01 containing recombinant Als3p and Hyr1p antigens for invasive Candida infections.
    Article Snippet: FluoroSpots in the unstimulated wells of each 787 mouse were subtracted from the antigen-stimulated spot counts and graphed.FluoroSpots in the unstimulated wells of each 787 mouse were subtracted from the antigen-stimulated spot counts and graphed.. 788 Infectious Inoculum Preparation 789 The C. albicans reference strain SC5314 (ATCC- MYA-2876) and C. auris strain 790 CAU-09 (South Asian clade, bronchoalveolar lavage [BAL]) were used in this AR TIC LE IN PR ES S 791 study.. These strains were grown in Yeast Extract Peptone Dextrose (YPD) broth 792 overnight at 30°C with shaking at 200 rpm.These strains were grown in Yeast Extract Peptone Dextrose (YPD) broth 792 overnight at 30°C with shaking at 200 rpm.

    Article Title: Next-generation Candida albicans vaccine VXV-01 containing recombinant Als3p and Hyr1p antigens for invasive Candida infections
    Article Snippet: The C. albicans reference strain SC5314 (ATCC- MYA-2876) and C. auris strain CAU-09 (South Asian clade, bronchoalveolar lavage [BAL]) were used in this study.. These strains were grown in Yeast Extract Peptone Dextrose (YPD) broth overnight at 30 °C with shaking at 200 rpm.These strains were grown in Yeast Extract Peptone Dextrose (YPD) broth overnight at 30 °C with shaking at 200 rpm.

    Article Title: Monoclonal antibodies targeting Candida disrupt biofilms and inhibit growth across global clinical isolates
    Article Snippet: Candida auris clinical isolates AR-0381 (CAU-01), AR-0382 (CAU-02), AR-0383 (CAU-03), AR-0384 (CAU-04), AR-0385 (CAU-05), AR-0386 (CAU-06), AR-0389 (CAU-09) were supplied by the United States Centers for Disease Control and Prevention (CDC, Atlanta, GA, USA).Candida auris clinical isolates AR-0381 (CAU-01), AR-0382 (CAU-02), AR-0383 (CAU-03), AR-0384 (CAU-04), AR-0385 (CAU-05), AR-0386 (CAU-06), AR-0389 (CAU-09) were supplied by the United States Centers for Disease Control and Prevention (CDC, Atlanta, GA, USA).. C. albicans reference strain SC5314 was supplied by the American Type Culture Collection (MYA-2876, ATCC, Manassas, VA, USA).. Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.

    Article Title: Liposomal Fba and Met6 peptide vaccination protects mice from disseminated candidiasis
    Article Snippet: C. auris clinical isolate AR-0389 (CAU-09) was supplied by the U.S. Centers for Disease Control and Prevention (CDC; Atlanta, GA, USA).C. auris clinical isolate AR-0389 (CAU-09) was supplied by the U.S. Centers for Disease Control and Prevention (CDC; Atlanta, GA, USA).. C. albicans reference strain SC5314 was supplied by the American Type Culture Collection (MYA-2876, ATCC, Manassas, VA, USA).. Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.

    Article Title: Liposomal Fba and Met6 peptide vaccination protects mice from disseminated candidiasis.
    Article Snippet: Candida isolates and culture conditions C. auris clinical isolate AR-0389 (CAU-09) was supplied by the U.S. Centers for Disease Control and Prevention (CDC; Atlanta, GA, USA).Candida isolates and culture conditions C. auris clinical isolate AR-0389 (CAU-09) was supplied by the U.S. Centers for Disease Control and Prevention (CDC; Atlanta, GA, USA).. C. albicans reference strain SC5314 was supplied by the American Type Culture Collection (MYA-2876, ATCC, Manassas, VA, USA).. Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.

    Article Title: Candida Cell-Surface-Specific Monoclonal Antibodies Protect Mice against Candida auris Invasive Infection
    Article Snippet: Two antifungal-resistant isolates of C. auris , AR-0386 (CAU-06) and AR-0389 (CAU-09), were supplied by the United States Centers for Disease Control and Prevention (CDC, Atlanta, GA, USA).Two antifungal-resistant isolates of C. auris , AR-0386 (CAU-06) and AR-0389 (CAU-09), were supplied by the United States Centers for Disease Control and Prevention (CDC, Atlanta, GA, USA).. C. albicans reference strain SC5314 was supplied by the American Type Culture Collection (MYA-2876, ATCC, Manassas, VA, USA).. For passive transfer of mAb experiments, the inoculum was serially passaged daily for three days in 25 mL glucose yeast peptide broth at 37 °C and then washed three times in Dulbecco’s phosphate-buffered saline (DPBS).For passive transfer of mAb experiments, the inoculum was serially passaged daily for three days in 25 mL glucose yeast peptide broth at 37 °C and then washed three times in Dulbecco’s phosphate-buffered saline (DPBS).

    Staining:

    Article Title: Nature-Derived Ferulic Acid Hybrids with Enhanced Antifungal and Antivirulence Activity Against Candida albicans .
    Article Snippet: C. albicans reference strain SC5314 (wild type; ATCC MYA-2876) was used throughout this study [38].. Yeast cells were routinely maintained on Sabouraud Dextrose Agar (SDA) plates and incubated at 37 ◦C for 24 h. For experimental assays, colonies were harvested and resuspended in liquid Sabouraud medium, followed by incubation at 37 ◦C under shaking conditions.Yeast cells were routinely maintained on Sabouraud Dextrose Agar (SDA) plates and incubated at 37 ◦C for 24 h. For experimental assays, colonies were harvested and resuspended in liquid Sabouraud medium, followed by incubation at 37 ◦C under shaking conditions.

    Article Title: Nature-Derived Ferulic Acid Hybrids with Enhanced Antifungal and Antivirulence Activity Against Candida albicans
    Article Snippet: C. albicans reference strain SC5314 (wild type; ATCC MYA-2876) was used throughout this study [ ].. Yeast cells were routinely maintained on Sabouraud Dextrose Agar (SDA) plates and incubated at 37 °C for 24 h. For experimental assays, colonies were harvested and resuspended in liquid Sabouraud medium, followed by incubation at 37 °C under shaking conditions.Yeast cells were routinely maintained on Sabouraud Dextrose Agar (SDA) plates and incubated at 37 °C for 24 h. For experimental assays, colonies were harvested and resuspended in liquid Sabouraud medium, followed by incubation at 37 °C under shaking conditions.

    Article Title: Next-generation Candida albicans vaccine VXV-01 containing recombinant Als3p and Hyr1p antigens for invasive Candida infections.
    Article Snippet: FluoroSpots in the unstimulated wells of each 787 mouse were subtracted from the antigen-stimulated spot counts and graphed.FluoroSpots in the unstimulated wells of each 787 mouse were subtracted from the antigen-stimulated spot counts and graphed.. 788 Infectious Inoculum Preparation 789 The C. albicans reference strain SC5314 (ATCC- MYA-2876) and C. auris strain 790 CAU-09 (South Asian clade, bronchoalveolar lavage [BAL]) were used in this AR TIC LE IN PR ES S 791 study.. These strains were grown in Yeast Extract Peptone Dextrose (YPD) broth 792 overnight at 30°C with shaking at 200 rpm.These strains were grown in Yeast Extract Peptone Dextrose (YPD) broth 792 overnight at 30°C with shaking at 200 rpm.

    Article Title: Next-generation Candida albicans vaccine VXV-01 containing recombinant Als3p and Hyr1p antigens for invasive Candida infections
    Article Snippet: The C. albicans reference strain SC5314 (ATCC- MYA-2876) and C. auris strain CAU-09 (South Asian clade, bronchoalveolar lavage [BAL]) were used in this study.. These strains were grown in Yeast Extract Peptone Dextrose (YPD) broth overnight at 30 °C with shaking at 200 rpm.These strains were grown in Yeast Extract Peptone Dextrose (YPD) broth overnight at 30 °C with shaking at 200 rpm.

    Article Title: Monoclonal antibodies targeting Candida disrupt biofilms and inhibit growth across global clinical isolates
    Article Snippet: Candida auris clinical isolates AR-0381 (CAU-01), AR-0382 (CAU-02), AR-0383 (CAU-03), AR-0384 (CAU-04), AR-0385 (CAU-05), AR-0386 (CAU-06), AR-0389 (CAU-09) were supplied by the United States Centers for Disease Control and Prevention (CDC, Atlanta, GA, USA).Candida auris clinical isolates AR-0381 (CAU-01), AR-0382 (CAU-02), AR-0383 (CAU-03), AR-0384 (CAU-04), AR-0385 (CAU-05), AR-0386 (CAU-06), AR-0389 (CAU-09) were supplied by the United States Centers for Disease Control and Prevention (CDC, Atlanta, GA, USA).. C. albicans reference strain SC5314 was supplied by the American Type Culture Collection (MYA-2876, ATCC, Manassas, VA, USA).. Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.

    Article Title: Liposomal Fba and Met6 peptide vaccination protects mice from disseminated candidiasis
    Article Snippet: C. auris clinical isolate AR-0389 (CAU-09) was supplied by the U.S. Centers for Disease Control and Prevention (CDC; Atlanta, GA, USA).C. auris clinical isolate AR-0389 (CAU-09) was supplied by the U.S. Centers for Disease Control and Prevention (CDC; Atlanta, GA, USA).. C. albicans reference strain SC5314 was supplied by the American Type Culture Collection (MYA-2876, ATCC, Manassas, VA, USA).. Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.

    Article Title: Liposomal Fba and Met6 peptide vaccination protects mice from disseminated candidiasis.
    Article Snippet: Candida isolates and culture conditions C. auris clinical isolate AR-0389 (CAU-09) was supplied by the U.S. Centers for Disease Control and Prevention (CDC; Atlanta, GA, USA).Candida isolates and culture conditions C. auris clinical isolate AR-0389 (CAU-09) was supplied by the U.S. Centers for Disease Control and Prevention (CDC; Atlanta, GA, USA).. C. albicans reference strain SC5314 was supplied by the American Type Culture Collection (MYA-2876, ATCC, Manassas, VA, USA).. Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.Yeast cells were grown overnight in 25 mL glucose yeast extract peptone (GYEP) broth in an orbital shaker at 37°C.

    Article Title: Candida Cell-Surface-Specific Monoclonal Antibodies Protect Mice against Candida auris Invasive Infection
    Article Snippet: Two antifungal-resistant isolates of C. auris , AR-0386 (CAU-06) and AR-0389 (CAU-09), were supplied by the United States Centers for Disease Control and Prevention (CDC, Atlanta, GA, USA).Two antifungal-resistant isolates of C. auris , AR-0386 (CAU-06) and AR-0389 (CAU-09), were supplied by the United States Centers for Disease Control and Prevention (CDC, Atlanta, GA, USA).. C. albicans reference strain SC5314 was supplied by the American Type Culture Collection (MYA-2876, ATCC, Manassas, VA, USA).. For passive transfer of mAb experiments, the inoculum was serially passaged daily for three days in 25 mL glucose yeast peptide broth at 37 °C and then washed three times in Dulbecco’s phosphate-buffered saline (DPBS).For passive transfer of mAb experiments, the inoculum was serially passaged daily for three days in 25 mL glucose yeast peptide broth at 37 °C and then washed three times in Dulbecco’s phosphate-buffered saline (DPBS).



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